Description
Rabbit polyclonal to HE4
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Human mature HE4 fused to glutathione S-transferase. Produced in E.coli.
Positive control
Normal human epididymis or human ovarian tumour.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, BSA
Concentration
- 100 µl at 0.03mg/ml
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
- Datasheet PDF
- SDS
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Immunohistochemistry （Formalin/PFA-fixed paraffin-embedded sections） - HE4 antibody （ab24480）
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Applications
Our Abpromise guarantee covers the use of ab24480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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WB
WB: 1/100. Detects a ban...Read more →WB: 1/100. Detects a band of approximay 25 kDa （predicted molecular weight: 13 kDa）.
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IHC-P
IHC-P: 1/40. Perform hea...Read more →IHC-P: 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Tissue specificity
Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
Sequence similarities
Contains 2 WAP domains.
Cellular localization
Secreted.
Form
There are 5 isoforms produced by alternative splicing.
Alternative names
- dJ461P17.6 antibody
- EDDM4 antibody
- epididymal protein 4 antibody
Database links
- Entrez Gene: 10406 Human
- SwissProt: Q14508 Human
- Unigene: 2719 Human
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